Category - Anal
The journals title analytical biochemistry methods in the biological sciences declares its broad scope methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would. Apart from full-length original research papers, the journal also welcomes review papers, and notes and tipsfeaturing methods that can be summarized in a shorter format article, including helpful kitchen tricks. Notes and tips for improving quality of lipid-protein overlay assays. Notes & tips reaction of tris(2-carboxyethyl)phosphine (tcep) with maleimide and -haloacyl groups anomalous elution of tcep by gel filtration author links open overlay panel douglas e. Notes & tips a simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells author links open overlay panel jean luc parrou jean françois. Can your protein be sumoylated? A quick summary and important tips to study sumo-modified proteins. And review of the resulting proof before it is published in its final citable form. 347 (2005) 152155 is not an issue, direct rca labeling and automated scan-ning of the arrays could be an assay of choice. In summary, we have reported that rca with 29 dna polymerase and cy3-labeled dntps is a simple tool for generation of high-density xuorescently labeled amplicons. Cafiso solutes modify a conformational transition in a membrane transport protein biophysical journal, 90, 2922-9, 2006. Notes and tips for improving quality of lipid-protein overlay assays. A novel inhibitor of homeodomain interacting protein kinase 2 mitigates kidney fibrosis through inhibition of the tgf-ß1smad3 pathway. Electrophoresis in polyacrylamide gels and transferred to nitrocellulose membranes (as described in anal. Ponceau s is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein.).